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KMID : 0613820130230010038
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Journal of Life Science
2013 Volume.23 No. 1 p.38 ~ p.43
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Study of Anti-inflammatory Effect of CopA3 Peptide Derived from Copris tripartitus
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Kim Hyeon-Jeong
Kim Dong-Hee
Lee Jin-Young
Hwang Jae-Sam
Lee Joon-Ha
Lee Seul-Gi
Jeong Hyeon-Guk
An Bong-Jeun
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Abstract
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The objective of this study was to evaluate the effect of the synthetic CopA3 peptide of Copris tripartitus on skin inflammation. Regulatory mechanisms of cytokines and nitric oxide (NO) are involved in the immunological activity of RAW 264.7 cells. Tested cells were treated with different concentrations of CopA3 and further cultured for an appropriate time after lipopolyssacharide (LPS) addition. During the entire experimental period, 5, 25, 50, and 100 μg/ml of CopA3 had no cytotoxicity. At these concentrations, CopA3 inhibited tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). CopA3 also inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). CopA3 inhibited the activity of iNOS and COX-2 by 41% and 59%, respectively, at 100 μg/ml. In addition, CopA3 reduced the release of inflammatory cytokines including TNF-α, IL-1β, and IL-6. These results suggest that CopA3 may have significant effects on inflammatory factors and that it may be a potential anti-inflammatory therapeutic agent.
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KEYWORD
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Anti-inflammatory, cyclooxygenase-2 (COX-2), inducible NO synthase (iNOS), CopA3, cytokine, Copris tripartitus
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